Fig. 7. SMARCB1 and HOTAIR overexpression restore SNAIL expression with genistein treatment. (a) 786-O and ACHN cells were treated with genistein (25 μM) or control (DMSO) for 96 hours and quantitative RT-PCR was performed for SNAIL and (b) expression of SNAIL protein was analyzed by Western blot. 786-O and ACHN cells in 12-well plates were treated with 25 uM genistein. After 24 hours, the cells were transfected with 25 pmol control siRNA or SMARCB1 siRNA and incubated for 72 hours in the presence of genistein. After 48 hours, the cells were transfected with 2.5 ng of HOTAIR expression vector for 48 hours in the presence of genistein. SMARCB1 proteins from 786-O and ACHN cells were pulled down using anti-SMARCB1 antibody or control IgG. (c) HOTAIR associated with SMARCB1 was detected by quantitative RT-PCR. Data from quantitative RT-PCR are presented with respect to IgG that is set to a value of 1. (d) Immunoprecipitated SMARCB1 was analyzed by Western blot. (e) SMARCB1 protein expression was analyzed by Western blot. (f) SNAIL mRNA expression was analyzed by quantitative RT-PCR. *p<0.05, **p<0.01, ***p<0.001. 'n.s.' p>0.05.